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Transgenic tobacco and peas expressing a proteinase inhibitor from Nicotiana alata have increased insect resistance. Molecular Breeding 5, 357-365.
| Charity J.A., Anderson, Marilyn A., Bittisnich, Dennis J., Whitecross, Malcolm, Higgins, T. J. V. (1999).
| Proteinase inhibitors have been used to increase resistance to insect pests in transgenic plants. A cDNA clone encoding a multi-domain proteinase inhibitor precursor from Nicotiana alata (Na-PI) was transferred into tobacco and peas under the control of a promoter from a ribulose-1, 5-bisphosphate carboxylase small subunit gene. The Na-PI precursor was cleaved in the leaves of transgenic tobacco and peas, and Mr 6000 polypeptides accumulated to levels of 0.3% and 0.1%, respectively, of the total soluble protein. The Na-PI cDNA segregated as a dominant Mendelian trait and was stably transmitted for at least two generations of both species. Helicoverpa armigera larvae that ingested tobacco or pea leaves containing Na-PI exhibited higher mortality or were delayed in growth and development relative to control larvae. A multi-domain proteinase inhibitor precursor from Nicotiana alata was transferred into tobacco and peas. Tobacco budworm (Helicoverpa armigera) larvae that ingested tobacco or pea leaves of these transgenic plants exhibited higher mortality or were delayed in growth and development relative to control larvae.
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