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Construction of vector with IMT1 and its gene expression in transgenic tobacco leaf cells associated with salt tolerance. Acta Botanica Sinica 41, 146-149.
| Dong Y.-Z. (1999).
| The plant expression vector with Imt1 (inositol O-methyltransferase) gene, pDH5, was constructed. The Imt1 gene was introduced to tobacco (Nicotiana tabacum cv. SR1) via Agrobacterium-mediated transformation. Thirty kanamycin-resistant shoots in MSS (MSO + 1 mg/L 6-BA + 30 g/L sucrose) selective medium was transferred to MSr (MSO + 3 g/L sucrose + 7 g/L glucose) medium supplemented with 1.2% approximates 1.5% NaCl for rooting. Eight plantlets rooted among the thirty shoots. PCR and Southern blot assay indicated that the Imt1 gene was integrated into tobacco genome in six plantlets. HPLC analysis showed that the product of Imt1 gene, D-ononitol accumulated in tobacco fresh leaf cells in a scope of 100 approximates 654 nmol/g FW. Western blot demonstrated the expression of Imt1 gene in tobacco cells.
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